%0 Journal Article
%T An intronic SNP affects skeletal muscle development by regulating the expression of TP63.
%A Chen Y
%A Wang Z
%A Qu X
%A Song B
%A Tang Y
%A Li B
%A Cao G
%A Yi G
%J Front Vet Sci
%V 11
%N 0
%D 2024
%M 38933706
%F 3.471
%R 10.3389/fvets.2024.1396766
%X <B>UNASSIGNED: </B>Porcine skeletal muscle development is pivotal for improving meat production. TP63, a transcription factor, regulates vital cellular processes, yet its role in skeletal muscle proliferation is unclear.<BR><B>UNASSIGNED: </B>The effects of TP63 on skeletal muscle cell viability and proliferation were investigated using both mouse and porcine skeletal muscle myoblasts. Selective sweep analysis in Western pigs identified TP63 as a potential candidate gene for skeletal muscle development. The correlation between TP63 overexpression and cell proliferation was assessed using quantitative real-time PCR (RT-qPCR) and 5-ethynyl-2'-deoxyuridine (EDU).<BR><B>UNASSIGNED: </B>The study revealed a positive correlation between TP63 overexpression and skeletal muscle cell proliferation. Bioinformatics analysis predicted an interaction between MEF2A, another transcription factor, and the mutation site of TP63. Experimental validation through dual-luciferase assays confirmed that a candidate enhancer SNP could influence MEF2A binding, subsequently regulating TP63 expression and promoting skeletal muscle cell proliferation.<BR><B>UNASSIGNED: </B>These findings offer experimental evidence for further exploration of skeletal muscle development mechanisms and the advancement of genetic breeding strategies aimed at improving meat production traits.