%0 Journal Article
%T Upscaling and Risk Evaluation of the Synthesis of the 3,5-Diamino-1H-Pyrazole, Disperazol.
%A Jansen CU
%A Grier KE
%A Andersen JB
%A Hultqvist LD
%A Nilsson M
%A Moser C
%A Graz M
%A Tolker-Nielsen T
%A Givskov M
%A Qvortrup K
%J Int J Mol Sci
%V 25
%N 12
%D 2024 Jun 19
%M 38928443
%F 6.208
%R 10.3390/ijms25126737
%X This paper presents the work performed to transition a lab-scale synthesis (1 g) to a large-scale (400 g) synthesis of the 3-5-diamino-1H-Pyrazole Disperazol, a new pharmaceutical for treatment of antibiotic-resistant Pseudomonas aeruginosa biofilm infections. The potentially hazardous diazotisation step in the lab-scale synthesis was transformed to a safe and easy-to-handle flow chemistry step. Additionally, the paper presents an OSHA-recommended safety assessment of active compound E, as performed by Fauske and Associates, LLC, Burr Ridge, IL, USA.