%0 Journal Article
%T Postweaning sodium citrate exposure induces long-lasting and sex-dependent effects on social behaviours in mice.
%A Qin Z
%A He X
%A Gao Q
%A Li Y
%A Zhang Y
%A Wang H
%A Qin N
%A Wang C
%A Huang B
%A Shi Y
%A Liu C
%A Wang S
%A Zhang H
%A Li Y
%A Shi H
%A Tian X
%A Song L
%J Pharmacol Biochem Behav
%V 242
%N 0
%D 2024 Sep 24
%M 38925482
%F 3.697
%R 10.1016/j.pbb.2024.173807
%X <B>BACKGROUND: </B>Postweaning is a pivotal period for brain development and individual growth. As an important chemical used in medicines, foods and beverages, sodium citrate (SC) is commonly available. Although some effects of SC exposure on individual physiology have been demonstrated, the potential long-lasting effects of postweaning dietary SC exposure on social behaviours are still elusive.<BR><B>METHODS: </B>Both postweaning male and female C57BL/6 mice were exposed to SC through drinking water for a total of 3 weeks. A series of behavioural tests, including social dominance test (SDT), social interaction test (SIT), bedding preference test (BPT) and sexual preference test (SPT), were performed in adolescence and adulthood. After these tests, serum oxytocin (OT) levels and gut microbiota were detected.<BR><B>RESULTS: </B>The behavioural results revealed that postweaning SC exposure decreased the social dominance of male mice in adulthood and female mice in both adolescence and adulthood. SC exposure also reduced the sexual preference rates of both males and females, while it had no effect on social interaction behaviour. ELISA results indicated that SC exposure decreased the serum OT levels of females but not males. 16S rRNA sequencing analysis revealed a significant difference in β-diversity after SC exposure in both males and females. The correlation coefficient indicated the correlation between social behaviours, OT levels and dominant genera of gut microbiota.<BR><B>CONCLUSIONS: </B>Our findings suggest that postweaning SC exposure may have enduring and sex-dependent effects on social behaviours, which may be correlated with altered serum OT levels and gut microbiota composition.