%0 Journal Article
%T CHCHD4 regulates the expression of mitochondrial genes that are essential for tumour cell growth.
%A Thomas LW
%A Stephen JM
%A Ashcroft M
%J Biochim Biophys Acta Mol Basis Dis
%V 1870
%N 7
%D 2024 10 21
%M 38909850
%F 6.633
%R 10.1016/j.bbadis.2024.167282
%X CHCHD4 (MIA40) is central to the functions of the mitochondrial disulfide relay system (DRS). CHCHD4 is essential and evolutionarily conserved. Previously, we have shown CHCHD4 to be a critical regulator of tumour cell growth. Here, we use integrated analysis of our genome-wide CRISPR/Cas9 and SILAC proteomic screening data to delineate mechanisms of CHCHD4 essentiality in cancer. We identify a shortlist of common essential genes/proteins regulated by CHCHD4, including subunits of complex I that are known DRS substrates, and genes/proteins involved in key metabolic pathways. Our study highlights a range of CHCHD4-regulated nuclear encoded mitochondrial genes/proteins essential for tumour cell growth.