%0 Journal Article
%T Genetic intratumour heterogeneity and clonal evolution in extramammary Paget's disease.
%A Tanaka K
%A Kajihara I
%A Shimokawa K
%A Nakamura N
%A Kusaba Y
%A Sakamoto R
%A Maeda-Otsuka S
%A Yamada-Kanazawa S
%A Sawamura S
%A Kanemaru H
%A Makino K
%A Aoi J
%A Masuguchi S
%A Suzuki T
%A Fukushima S
%J Eur J Dermatol
%V 34
%N 2
%D 2024 Apr 1
%M 38907549
%F 2.805
%R 10.1684/ejd.2024.4609
%X All tumour cells in a patient have shared and non-shared genetic alterations, and the diversity of mutations is described as intratumoural heterogeneity (ITH). Multiregion sequencing is a genome sequencing analytical technique used for multiple, spatially-separated biopsy tissues that may further our understanding of ITH and tumour evolution. Although genetic mutations in extramammary Paget's disease (EMPD) have recently been detected by next-generation sequencing analysis, there have been no reports of ITH based on multiregion sequencing in EMPD. Thus, we clarified the landscape of ITH and tumour evolution in EMPD. We performed whole-exome sequencing on 35 tissues (30 tumour tissues and five normal skin samples as a paired control), collected from five patients with EMPD. The rate of private mutations was significantly higher than that of ubiquitous and shared mutations. Ubiquitous mutations were not present in driver genes, and most driver genes exhibited private and shared mutations. The most frequent base substitution was C>T in almost all lesions, and most mutational signatures corresponded to signature 1, 2, 3, and 8. The types of proposed aetiology in most lesions were based on age and AID/APOBEC family and BRCA1/BRCA2 mutations. Evolutionary trees were characterized by short trunks and long branches due to the extremely high ratio of private mutations. In contrast, pathogenic factors, such as base substitutions, mutational signatures, and proposed aetiology, were shared. Tumour evolution in EMPD appears to be characterized by a high level of genetic ITH with shared background factors.