%0 Journal Article
%T A targeted LC-MRM3 proteomic approach for the diagnosis of SARS-CoV-2 infection in nasopharyngeal swabs.
%A Drouin N
%A Elfrink HL
%A Boers SA
%A van Hugten S
%A Wessels E
%A de Vries JJC
%A Groeneveld GH
%A Miggiels P
%A Van Puyvelde B
%A Dhaenens M
%A Budding AE
%A Ran L
%A Masius R
%A Takats Z
%A Boogaerds A
%A Bulters M
%A Muurlink W
%A Oostvogel P
%A Harms AC
%A van der Lubben M
%A Hankemeier T
%J Mol Cell Proteomics
%V 0
%N 0
%D 2024 Jun 17
%M 38897290
暂无%R 10.1016/j.mcpro.2024.100805
%X Since its first appearance, SARS-CoV-2 quickly spread around the world and the lack of adequate PCR testing capacities, especially during the early pandemic, led the scientific community to explore new approaches such as mass spectrometry (MS). We developed a proteomics workflow to target several tryptic peptides of the nucleocapsid protein (NCAP). A highly selective multiple reaction monitoring MRM3 strategy provided a sensitivity increase in comparison to conventional MRM acquisition. Our MRM3 approach was first tested on an Amsterdam public health cohort (alpha-variant, 760 participants) detecting viral NCAP peptides from nasopharyngeal swabs samples presenting a cycle threshold (Ct) value down to 35 with sensitivity and specificity of 94.2% and 100.0%, without immuno-purification. A second iteration of the MS-diagnostic test, able to analyze more than 400 samples per day, was clinically validated on a Leiden-Rijswijk public health cohort (delta-variant, 2536 participants) achieving 99.9% specificity and 93.1% sensitivity for patients with Ct-values up to 35. In this manuscript, we also developed and brought the first proof of the concept of viral variant monitoring in a complex matrix using targeted mass spectrometry.