%0 Journal Article
%T NDT-C11 as a Viable Novel Detergent for Single Particle Cryo-EM.
%A Jiko C
%A Li J
%A Moon Y
%A Tanaka Y
%A Gopalasingam CC
%A Shigematsu H
%A Chae PS
%A Kurisu G
%A Gerle C
%J Chempluschem
%V 0
%N 0
%D 2024 Jun 17
%M 38881532
%F 3.21
%R 10.1002/cplu.202400242
%X Single particle cryo electron microscopy (cryo-EM) is now the major method for the determination of integral membrane protein structure. For the success of a given project the type of membrane mimetic used for extraction from the native cell membrane, purification to homogeneity and finally cryo-grid vitrification is crucial. Although small molecule amphiphiles - detergents - are the most widely used membrane mimetic, specific tailoring of detergent structure for single particle cryo-EM is rare and the demand for effective detergents not satisfied. Here, we compare the popular detergent lauryl maltose-neopentyl glycol (LMNG) with the novel detergent neopentyl glycol-derived triglucoside-C11 (NDT-C11) in its behavior as free detergent and when bound to two types of multisubunit membrane protein complexes - cyanobacterial photosystem I (PSI) and mammalian F-ATP synthase. We conclude that NDT-C11 has high potential to become a very useful detergent for single particle cryo-EM of integral membrane proteins.