%0 Journal Article
%T Single-cell rapid identification, in situ viability and vitality profiling, and genome-based source-tracking for probiotics products.
%A Zhang J
%A Ren L
%A Zhang L
%A Gong Y
%A Xu T
%A Wang X
%A Guo C
%A Zhai L
%A Yu X
%A Li Y
%A Zhu P
%A Chen R
%A Jing X
%A Jing G
%A Zhou S
%A Xu M
%A Wang C
%A Niu C
%A Ge Y
%A Ma B
%A Shang G
%A Cui Y
%A Yao S
%A Xu J
%J Imeta
%V 2
%N 3
%D 2023 Aug
%M 38867931
暂无%R 10.1002/imt2.117
%X Rapid expansion of the probiotics industry demands fast, sensitive, comprehensive, and low-cost strategies for quality assessment. Here, we introduce a culture-free, one-cell-resolution, phenome-genome-combined strategy called Single-Cell Identification, Viability and Vitality tests, and Source-tracking (SCIVVS). For each cell directly extracted from the product, the fingerprint region of D2O-probed single-cell Raman spectrum (SCRS) enables species-level identification with 93% accuracy, based on a reference SCRS database from 21 statutory probiotic species, whereas the C-D band accurately quantifies viability, metabolic vitality plus their intercellular heterogeneity. For source-tracking, single-cell Raman-activated Cell Sorting and Sequencing can proceed, producing indexed, precisely one-cell-based genome assemblies that can reach ~99.40% genome-wide coverage. Finally, we validated an integrated SCIVVS workflow with automated SCRS acquisition where the whole process except sequencing takes just 5 h. As it is >20-fold faster, >10-time cheaper, vitality-revealing, heterogeneity-resolving, and automation-prone, SCIVVS is a new technological and data framework for quality assessment of live-cell products.