%0 Journal Article
%T Endosymbiosis in trypanosomatids: The bacterium division depends on microtubule dynamism.
%A Moraes JR
%A Barrinha A
%A Gonçalves de Lima LS
%A Vidal JC
%A Costa Catta-Preta CM
%A de Souza W
%A Zuma AA
%A Motta MCM
%J Exp Cell Res
%V 440
%N 1
%D 2024 Jul 1
%M 38857838
%F 4.145
%R 10.1016/j.yexcr.2024.114126
%X Microtubules are components of the cytoskeleton that perform essential functions in eukaryotes, such as those related to shape change, motility and cell division. In this context some characteristics of these filaments are essential, such as polarity and dynamic instability. In trypanosomatids, microtubules are integral to ultrastructure organization, intracellular transport and mitotic processes. Some species of trypanosomatids co-evolve with a symbiotic bacterium in a mutualistic association that is marked by extensive metabolic exchanges and a coordinated division of the symbiont with other cellular structures, such as the nucleus and the kinetoplast. It is already established that the bacterium division is microtubule-dependent, so in this work, it was investigated whether the dynamism and remodeling of these filaments is capable of affecting the prokaryote division. To this purpose, Angomonas deanei was treated with Trichostatin A (TSA), a deacetylase inhibitor, and mutant cells for histone deacetylase 6 (HDAC6) were obtained by CRISPR-Cas9. A decrease in proliferation, an enhancement in tubulin acetylation, as well as morphological and ultrastructural changes, were observed in TSA-treated protozoa and mutant cells. In both cases, symbiont filamentation occurred, indicating that prokaryote cell division is dependent on microtubule dynamism.