%0 Journal Article
%T Altered nucleocytoplasmic export of adenosine-rich circRNAs by PABPC1 contributes to neuronal function.
%A Cao SM
%A Wu H
%A Yuan GH
%A Pan YH
%A Zhang J
%A Liu YX
%A Li S
%A Xu YF
%A Wei MY
%A Yang L
%A Chen LL
%J Mol Cell
%V 84
%N 12
%D 2024 Jun 20
%M 38838666
%F 19.328
%R 10.1016/j.molcel.2024.05.011
%X Circular RNAs (circRNAs) are upregulated during neurogenesis. Where and how circRNAs are localized and what roles they play during this process have remained elusive. Comparing the nuclear and cytoplasmic circRNAs between H9 cells and H9-derived forebrain (FB) neurons, we identify that a subset of adenosine (A)-rich circRNAs are restricted in H9 nuclei but exported to cytosols upon differentiation. Such a subcellular relocation of circRNAs is modulated by the poly(A)-binding protein PABPC1. In the H9 nucleus, newly produced (A)-rich circRNAs are bound by PABPC1 and trapped by the nuclear basket protein TPR to prevent their export. Modulating (A)-rich motifs in circRNAs alters their subcellular localization, and introducing (A)-rich circRNAs in H9 cytosols results in mRNA translation suppression. Moreover, decreased nuclear PABPC1 upon neuronal differentiation enables the export of (A)-rich circRNAs, including circRTN4(2,3), which is required for neurite outgrowth. These findings uncover subcellular localization features of circRNAs, linking their processing and function during neurogenesis.