%0 Journal Article
%T Sinusoidal alternating electromagnetic field accelerates fracture healing in rats.
%A Gao Y
%A Hou X
%A Wei Z
%A Chen K
%J Zhejiang Da Xue Xue Bao Yi Xue Ban
%V 0
%N 0
%D 2024 Jun 4
%M 38832464
暂无%R 10.3724/zdxbyxb-2023-0454
%X <B>OBJECTIVE: </B>To investigate the effect of sinusoidal alternating electromagnetic field (SEMF) on fracture healing and its mechanism.<BR><B>METHODS: </B>Femoral fracture model was established using SPF male Wistar rats, 30 model rats were randomly divided into model control (MC) and SEMF groups with 15 rats in each group. The SEMF group was given 50 Hz 1.8 mT for 90 min every day, and the MC group was not treated. X-ray examinations were performed every two weeks to determine the formation of bone scabs in each group of rats. Three rats were sacrificed after 2 and 4 weeks of treatment in both groups. Protein was extracted from the fractured femurs, and the expression of type Ⅰ collagen (COL-1), Osterix (OSX), Runt-related transcription factor 2 (RUNX2), and vascular endothelial growth factor (VEGF) protein level was detected by immunoblotting. After 8 weeks, the femur on the operated side was taken for micro-CT scanning to observe fracture healing, angiography to observe blood vessel growth, and organs such as hearts, livers, spleens, lungs, and kidneys were taken for safety evaluation by hematoxylin-eosin staining (HE staining).<BR><B>RESULTS: </B>The bone scab scores of the SEMF group were significantly higher than those of the MC group after 2, 4, 6, and 8 weeks of treatment (all P<0.01); the fracture healing of the SEMF group was better than that of the MC group after 8 weeks, and the bone volume scores of the two groups were 0.243±0.012 and 0.186±0.008, respectively, with statistically significant differences (P<0.01); and the number of blood vessels in the SEMF group was also more than that of the MC group after 8 weeks. The results of protein blotting method showed that the protein expression of VEGF, COL-1, RUNX2, and OSX was higher in the SEMF group than in the MC group after 2 and 4 weeks of treatment (all P<0.05), and the HE staining showed that there was no abnormality in histopathological observation of examined organs in both groups.<BR><B>CONCLUSIONS: </B>SEMF can accelerate fracture healing by promoting the expression of osteogenic factors and vascular proliferation without significant adverse effects.<BR>目的: 观察正弦交变电磁场（SEMF）对骨折愈合的影响并探讨其影响骨折愈合的作用机制。方法: 30只无特定病原体级雄性Wistar大鼠建立骨折模型后按随机分组法分为模型对照组和SEMF组，每组15只。SEMF组每天给予50 Hz 1.8 mT的SEMF 90 min，模型对照组不做任何处理。每两周进行X射线检查判断各组大鼠骨痂形成情况，在电磁场干预后的第二周和第四周各组分别处死3只大鼠并取术侧骨折处骨组织提取蛋白，蛋白质印迹法检测Ⅰ型胶原蛋白（COL-1）、Osterix蛋白（OSX）、Runt相关转录因子2（RUNX2）、血管内皮生长因子（VEGF）蛋白的表达。8周后取术侧股骨行显微CT扫描观察骨折愈合、行血管造影观察血管生长情况，并取心、肝、脾、肺、肾等器官采用苏木精-伊红染色（HE染色）进行安全性评价。结果: SEMF组治疗2、4、6、8周后骨痂评分均显著高于模型对照组（均P<0.01）；8周后SEMF组骨折愈合情况优于模型对照组，两组骨体积分数分别为0.243±0.012和0.186±0.008，差异有统计学意义（P<0.01）；8周后SEMF组血管数也均优于模型对照组。蛋白质印迹法结果显示，SEMF组治疗2、4周后VEGF、COL-1、RUNX2、OSX蛋白表达均高于模型对照组（均P<0.05）。HE染色结果显示，SEMF组和模型对照组各器官组织病理学观察均无异常。结论: SEMF能够通过促进不同时期成骨因子的蛋白表达和血管增生从而加速骨折的愈合，并且无明显不良反应。.