%0 Journal Article
%T Protocol for the cryopreservation of Cryptosporidium isolates using enteroids.
%A Deng M
%A Wei Y
%A Li N
%A Xiao L
%A Feng Y
%A Guo Y
%J STAR Protoc
%V 5
%N 2
%D 2024 Jun 21
%M 38824642
暂无%R 10.1016/j.xpro.2024.103101
%X A major bottleneck in the progress of Cryptosporidium research is the lack of accessible cryopreservation of Cryptosporidium oocysts. Here, we present a protocol for the cryopreservation of Cryptosporidium isolates using enteroids. We describe the steps for the establishment of enteroid cultures and cryopreservation of C. parvum-infected HCT-8 cultures. We then detail procedures for the recovery and propagation of frozen parasites using enteroids. For complete details on the use and execution of this protocol, please refer to Deng et al.1.