%0 Journal Article
%T Allergenicity and Linear Epitope Analysis of Scy p 8, an Allergen from Mud Crab.
%A Xia F
%A Li M
%A Liu Q
%A Liu H
%A Yang Y
%A Liu M
%A Chen G
%A Luo L
%A Liu Y
%A Liu G
%J J Agric Food Chem
%V 72
%N 23
%D 2024 Jun 12
%M 38821040
%F 5.895
%R 10.1021/acs.jafc.4c02693
%X Scy p 8 (triosephosphate isomerase) as a crab allergen in inducing distinct T-helper (Th) cell differentiation and a linear epitope associated with allergenicity remain elusive. In this study, mice sensitized with Scy p 8 exhibited significantly upregulated levels of IgE, IgG1, and IL-4 release, inducing a Th2 immune response. Moreover, the release of IFN-γ (Th1) and the levels of Treg cells were downregulated, while IL-17A (Th17) was upregulated, indicating that Scy p 8 disrupted the Th1/Th2 balance and Th17/Treg balance in mice. Furthermore, bioinformatics prediction and serum samples from crab-allergic patients and mice enabled the discovery of 8 linear epitopes of Scy p 8. Meanwhile, the analysis of peptide similarity and tertiary superposition revealed that 8 epitopes of Scy p 8 exhibited conservation across various species, potentially resulting in cross-reactivity. These findings possess the potential to enhance the comprehension of crab allergens, thereby establishing a foundation for investigating cross-reactivity.