%0 Journal Article
%T A Reference Profile of N-Glycosylation for Human Kidney and the Identification of Cell-Cell Interactions between Parietal Epithelial Cells and Capillary Endothelial Cells by Single-Cell Glycosylation-Sequencing.
%A Xiao M
%A Yan Q
%A Luan S
%A Lai L
%A Xu Z
%A Zou Y
%A Zeng Z
%A Li H
%A Qiu J
%A Tang D
%A Yin L
%A Dai Y
%J Kidney Blood Press Res
%V 49
%N 1
%D 2024 May 24
%M 38797171
%F 3.096
%R 10.1159/000539514
%X <B>BACKGROUND: </B>N-glycosylation is one of the most common posttranslational modifications in humans, and these alterations are associated with kidney diseases.<BR><B>METHODS: </B>A novel technological approach, single-cell N-acetyllactosamine sequencing (scLacNAc-seq), was applied to simultaneously detect N-glycosylation expression and the transcriptome at single-cell resolution in three human kidney tissues from zero-time biopsy. Cell clusters, glycation abundance in each cell cluster, functional enrichment analysis, cell-cell crosstalk, and pseudotime analysis were applied.<BR><B>RESULTS: </B>Using scLacNAc-seq, 24,247 cells and 22 cell clusters were identified, and N-glycan abundance in each cell was obtained. Transcriptome analysis revealed a close connection between capillary endothelial cells (CapECs) and parietal epithelial cells (PECs). PECs and CapECs communicate with each other through several pairs of ligand receptors (e.g., TGFB1-EGFR, GRN-EGFR, TIMP1-FGFR2, VEGFB-FLT1, ANGPT2-TEK, and GRN-TNFRSF1A). Finally, a regulatory network of cell-cell crosstalk between PECs and CapECs was constructed, which is involved in cell development.<BR><B>CONCLUSIONS: </B>We here, for the first time, constructed the glycosylation profile of 22 cell clusters in the human kidney from zero-time biopsy. Moreover, cell-cell communication between PECs and CapECs through the ligand-receptor system may play a crucial regulatory role in cell proliferation.