%0 Journal Article
%T A real-time biochemical assay for quantitative analyses of APOBEC-catalyzed DNA deamination.
%A Belica CA
%A Carpenter MA
%A Chen Y
%A Brown WL
%A Moeller NH
%A Boylan IT
%A Harris RS
%A Aihara H
%J J Biol Chem
%V 300
%N 6
%D 2024 Jun 23
%M 38796062
暂无%R 10.1016/j.jbc.2024.107410
%X Over the past decade, the connection between APOBEC3 cytosine deaminases and cancer mutagenesis has become increasingly apparent. This growing awareness has created a need for biochemical tools that can be used to identify and characterize potential inhibitors of this enzyme family. In response to this challenge, we have developed a Real-time APOBEC3-mediated DNA Deamination assay. This assay offers a single-step set-up and real-time fluorescent read-out, and it is capable of providing insights into enzyme kinetics. The assay also offers a high-sensitivity and easily scalable method for identifying APOBEC3 inhibitors. This assay serves as a crucial addition to the existing APOBEC3 biochemical and cellular toolkit and possesses the versatility to be readily adapted into a high-throughput format for inhibitor discovery.