%0 Journal Article
%T Moment of induction and duration of experimental varicocele in rats: effects on semen quality.
%A de Carvalho RC
%A Andretta RR
%A de Moura JAC
%A Bertolla RP
%A Okada FK
%J Int Braz J Urol
%V 50
%N 4
%D 2024 Jul-Aug
%M 38787514
%F 3.05
%R 10.1590/S1677-5538.IBJU.2023.0412
%X <B>OBJECTIVE: </B>Varicocele is a condition known to cause damage to seminal parameters and sperm function. Furthermore, it has been hypothesized that the varicocele effect on fertility is time-dependent; however, little is known about the consequences of its establishment time on reproductive organs and/or sperm function. This study aimed to evaluate the effect of the duration of experimental varicocele on reproductive organs, sperm parameters, and sperm function.<BR><B>METHODS: </B>Varicocele induction surgeries were performed in Wistar rats aged 40 or 100 days old. At 160-day-old, analyses were performed, including biometry of reproductive organs (prostate, seminal vesicles, epididymis, and testis), sperm parameters (vitality, morphology, and motility), and sperm function tests (nuclear DNA integrity, acrosome integrity, and mitochondrial activity).<BR><B>RESULTS: </B>The analysis of the biometry of reproductive organs showed no differences between distinct ages in which varicocele was induced. The total abnormal sperm morphology was bigger in animals with varicocele induced to 100 days old than in animals with varicocele induced to 40 days old. Regarding nuclear DNA integrity, animals of varicocele induced to 100 days old showed worse results compared to animals of varicocele induced to 40 days old. Other parameters analyzed showed no differences between varicocele groups.<BR><B>CONCLUSIONS: </B>In this study conducted on rats, we conclude that varicocele adversely affects sperm, particularly its function. However, we did not observe a negative progressive effect on sperm.