%0 Journal Article
%T AMPK inhibits voltage-gated calcium channel-current in rat chromaffin cells.
%A Fukumoto-Inukai AK
%A Bermeo K
%A Arenas I
%A Rosendo-Pineda MJ
%A Pimentel-Cabrera JA
%A Garcia DE
%J Mol Cell Endocrinol
%V 591
%N 0
%D 2024 Sep 15
%M 38777212
%F 4.369
%R 10.1016/j.mce.2024.112275
%X Metabolic changes are critical in the regulation of Ca2+ influx in central and peripheral neuroendocrine cells. To study the regulation of L-type Ca2+ channels by AMPK we used biochemical reagents and ATP/glucose-concentration manipulations in rat chromaffin cells. AICAR and Compound-C, at low concentration, significantly induce changes in L-type Ca2+ channel-current amplitude and voltage dependence. Remarkably, an overlasting decrease in the channel-current density can be induced by lowering the intracellular level of ATP. Accordingly, Ca2+ channel-current density gradually diminishes by decreasing the extracellular glucose concentration. By using immunofluorescence, a decrease in the expression of CaV1.2 is observed while decreasing extracellular glucose, suggesting that AMPK reduces the number of functional Ca2+ channels into the plasma membrane. Together, these results support for the first time the dependence of metabolic changes in the maintenance of Ca2+ channel-current by AMPK. They reveal a key step in Ca2+ influx in secretory cells.