%0 Journal Article
%T Single-cell proteomics and transcriptomics capture eosinophil development and identify the role of IL-5 in their lineage transit amplification.
%A Jorssen J
%A Van Hulst G
%A Mollers K
%A Pujol J
%A Petrellis G
%A Baptista AP
%A Schetters S
%A Baron F
%A Caers J
%A Lambrecht BN
%A Dewals BG
%A Bureau F
%A Desmet CJ
%J Immunity
%V 57
%N 7
%D 2024 Jul 9
%M 38776917
%F 43.474
%R 10.1016/j.immuni.2024.04.027
%X The activities, ontogeny, and mechanisms of lineage expansion of eosinophils are less well resolved than those of other immune cells, despite the use of biological therapies targeting the eosinophilia-promoting cytokine interleukin (IL)-5 or its receptor, IL-5Rα. We combined single-cell proteomics and transcriptomics and generated transgenic IL-5Rα reporter mice to revisit eosinophilopoiesis. We reconciled human and murine eosinophilopoiesis and provided extensive cell-surface immunophenotyping and transcriptomes at different stages along the continuum of eosinophil maturation. We used these resources to show that IL-5 promoted eosinophil-lineage expansion via transit amplification, while its deletion or neutralization did not compromise eosinophil maturation. Informed from our resources, we also showed that interferon response factor-8, considered an essential promoter of myelopoiesis, was not intrinsically required for eosinophilopoiesis. This work hence provides resources, methods, and insights for understanding eosinophil ontogeny, the effects of current precision therapeutics, and the regulation of eosinophil development and numbers in health and disease.