%0 Journal Article
%T Immunochromatography Lateral Flow Strip Enhancement Based on Passive Gold Nanoparticles Conjugation to Detect Schistosma haematobium Antigens in Human Serum.
%A El-Shall MN
%A Aly I
%A Samen A
%A Salama WM
%A Baakdah F
%J Acta Parasitol
%V 69
%N 2
%D 2024 Jun 16
%M 38753102
%F 1.534
%R 10.1007/s11686-024-00841-y
%X <B>OBJECTIVE: </B>This study aimed to develop and evaluate a lateral flow card for the detection of active Schistosoma haematobium infection.<BR><B>METHODS: </B>In order to prepare the immunochromatography lateral flow strip (ICLFS), antibodies purified from schistosomiasis were conjugated passively with gold nanoparticles using a potassium carbonate buffer.<BR><B>RESULTS: </B>The novel ICLFS was able to correctly identify 64 out of 67 samples of schistosomiasis, 6 out of 90 samples of other parasites, and 0 out of 27 control samples. Sensitivity, specificity, negative predictive value (NPV), and positive predictive value (PPV) were 95.5%, 93.3%, 90%, and 91.4% respectively. Comparatively, the sensitivity, specificity, NPV, and PPV of sandwich enzyme-linked immunosorbent assays (ELISA) conjugated with gold nanoparticles (AuNPs) were 91.1%, 88.8%, 85.9%, and 84.4% respectively. The increased sensitivity and specificity of ICLFS produced superior results to those of sandwich ELISA.<BR><B>CONCLUSIONS: </B>In conclusion, ICLFS is more beneficial and precise than sandwich ELISA for detection of S. haematobium infection at early stage.