%0 Journal Article
%T Histopathological Analysis of Brains from Dogs Infected with Canine Distemper Virus.
%A Verdes JM
%A Larrañaga C
%A Varela B
%A Iribarnegaray V
%A Yozzi V
%A Feijóo G
%A Yamasaki K
%J Methods Mol Biol
%V 2808
%N 0
%D 2024
%M 38743371
暂无%R 10.1007/978-1-0716-3870-5_14
%X We describe the use of conventional histology and immunohistochemistry against canine distemper virus (CDV) to examine the brains of domestic dogs with a confirmed diagnosis of CDV infection. Histologically, to identify the main typical lesions, we used conventional H&E stain; to evaluate the progressive demyelination, we used Luxol Fast Blue stain; and to identify the presence of viral particles in these affected regions, we used immunohistochemistry against CDV. We confirm that the histopathological analysis of brains of distemper-infected dogs is a powerful tool to evaluate the typical brain lesions and could be used as an interesting natural model to continue studying the pathogenesis of canine distemper in different species and/or other morbillivirus infections, like measles.