%0 Journal Article
%T Generate an AZFa deleted human embryonic stem cell line.
%A Zhen Y
%A He Q
%A Sun C
%A Ma Y
%A Li Q
%A Wen L
%J Stem Cell Res
%V 77
%N 0
%D 2024 Jun 3
%M 38733811
%F 1.587
%R 10.1016/j.scr.2024.103436
%X Y chromosome deletion and karyotype abnormalities are commonly associated with congenital non-obstructive azoospermia, impairing spermatogenesis. Specifically, the deletion of the Y chromosome Azoospermia factor a (AZFa) has been identified in infertile males with severely impaired spermatogenesis. AZFa, encompassing megabase-scale of the Y chromosome region, poses challenges in modeling AZFa deletion-related male infertility using gene editing tools. Here, we successfully created an AZFa-deleted human embryonic stem cell line utilizing the CRISPR/Cas9 gene editing tool. Our analysis indicates the AZFa-deleted stem cell line holds promise for differentiation into ectoderm, mesoderm, and endoderm, highlighting its potential for further comprehensive study.