%0 Journal Article
%T Construction and application of 3-fucosyllactose whole-cell biosensor for high-throughput screening of overproducers.
%A Li Q
%A Liu C
%A He J
%A Liu T
%A Zhang W
%A Xie Z
%A Zong J
%A Li Y
%A Sun X
%A Lu F
%J Bioresour Technol
%V 402
%N 0
%D 2024 Jun 3
%M 38705212
%F 11.889
%R 10.1016/j.biortech.2024.130798
%X Biosensor-based high-throughput screening is efficient for improving industrial microorganisms. There is a severe shortage of human milk oligosaccharides (HMOs) biosensors. This study established a 3-fucosyllactose (3-FL, a kind of HMOs) whole-cell biosensor by coupling cell growth with production. To construct and optimize the biosensor, an Escherichia coli 3-FL producer was engineered by deleting the manA, yihS and manX genes, directing the mannose flux solely to 3-FL synthesis. Then, an α-L-fucosidase was introduced to hydrolyze 3-FL to fucose which was used as the only carbon source for cell growth. Using the biosensor, the 3-FL production of a screened mutant was improved by 25 % to 42.05 ± 1.28 g/L. The productivity reached 1.17 g/L/h, the highest level reported by now. The csrB mutant obtained should be a new clue for the 3-FL overproduction mechanism. In summary, this study provided a novel approach to construct HMOs biosensors for strain improvement.