%0 Journal Article
%T Biochemical Properties and Immunogenic Epitopes of Echinococcus granulosus Glutathione S-Transferase as a Vaccine Target: In-Silico Study.
%A Khazaei S
%A Dalimi A
%A Pirestani M
%A Ghafarifar F
%J Iran J Parasitol
%V 19
%N 1
%D 2024 Jan-Mar
%M 38654954
%F 1.217
%R 10.18502/ijpa.v19i1.15205
%X <B>UNASSIGNED: </B>The current in silico study was done to determine the primary biochemical features and immunogenic epitopes of Echinococcus granulosus glutathione S-transferase protein as a potential vaccine candidate.<BR><B>UNASSIGNED: </B>Several web tools were employed to predict physico-chemical properties, antigenicity, allergenicity, solubility, post-translational modification (PTM) sites, subcellular localization, signal peptide, transmembrane domain, secondary and tertiary structure followed by refinement and validations. In addition, B-cell epitopes were predicted and were screened using various web servers, while MHC-binding and CTL epitopes were predicted using IEDB and NetCTL servers, respectively.<BR><B>UNASSIGNED: </B>The protein had 219 residues with a molecular weight of 25.55 kDa and alkaline isoelectric pH (7.5). This protein was stable, thermo-tolerant (aliphatic index: 78.04) and hydrophilic (GRAVY: -0.440). The predicted antigenicity scores were low and the protein was nonallergenic in nature. There were no transmembrane domain and signal peptide in the sequence. Moreover, several B-cell, MHC-binding and CTL epitopes were found in the EgGST protein, which could be further used in multi-epitope vaccines.<BR><B>UNASSIGNED: </B>Further studies are needed on the development of vaccines in vivo using EgGST alone or in combination with other antigens in the future.