%0 Journal Article
%T Chemical cross-linking to study protein self-assembly in cellulo.
%A Müller L
%A Salman S
%A Hoppe T
%J STAR Protoc
%V 5
%N 2
%D 2024 Jun 21
%M 38652664
暂无%R 10.1016/j.xpro.2024.103032
%X Many proteins self-assemble into dimers and higher-order oligomers. Therefore, the goal of this protocol is to characterize the conformational states of an endogenous protein of interest. Here, we present a protocol for assessing protein self-assembly in cell lysates using chemical cross-linking. We describe steps for chemical cross-linking with recombinant proteins as well as steps for cell culture and cell lysate preparation, chemical cross-linking, SDS-PAGE, and western blotting for the detection of endogenous proteins. For complete details on the use and execution of this protocol, please refer to Balaji et al.1.