%0 English Abstract
%T [Six-sequence-tagged site (STS) versus eight-STS scheme for detection of Y chromosome microdeletions].
%A Tian H
%A Shao MJ
%A Yan LY
%A Hong K
%A Qiao J
%J Zhonghua Nan Ke Xue
%V 29
%N 4
%D 2023 Apr
%M 38598213
暂无%X <B>OBJECTIVE: </B>To compare the six-sequence-tagged site (STS) with the eight-STS scheme in the detection of Y chromosome microdeletions.<BR><B>METHODS: </B>Using real-time quantitative PCR, we compared the results of the six-STS (sY84, sY86, sY127, sY134, sY254, sY255) scheme with those of the eight-STS (sY84, sY86, sY127, sY134, sY254, sY255, sY145, sY152) scheme in detecting Y chromosome microdeletions.<BR><B>RESULTS: </B>No statistically significant difference was found in the detection rate of the deletion of the azoospermia factor (AZF) regions between the six-STS and eight-STS methods (9.34% ［575/6177］ vs 8.85% ［542/6122］, P > 0.05).<BR><B>CONCLUSIONS: </B>Though the eight-STS scheme increased the detection of AZFd, its detection rate of the AZF region deletion was not significantly different from that of the six-STS method. From the perspectives of experimental operation, economic cost and clinical strategy guidance, the six-STS is better than the eight-STS scheme for the detection of Y chromosome microdeletions.