%0 Journal Article
%T Establishment and application of a reverse dot blot assay for 13 mutations of hearing-loss genes in primary hospitals in China.
%A Jiang QQ
%A Zhu JJ
%A Fan SL
%A Hou YP
%A Hu XY
%A Shi J
%A Wu L
%A Luo Y
%J Asian Biomed (Res Rev News)
%V 18
%N 1
%D 2024 Feb
%M 38515630
%F 1.017
%R 10.2478/abm-2024-0003
%X <B>UNASSIGNED: </B>Hearing loss is a common sensorineural dysfunction with a high incidence in China. Although genetic factors are important causes of hearing loss, hearing-related gene detection has not been widely adopted in China.<BR><B>UNASSIGNED: </B>Establishing a rapid and efficient method to simultaneously detect hotspot hearing loss gene mutations.<BR><B>UNASSIGNED: </B>A reverse dot blot assay combined with a flow-through hybridization technique was developed for the simultaneous detection of 13 hotspot mutations of 4 hearing loss-related genes including GJB2, GJB3, SLC26A4, and the mitochondrial gene MT-RNR1. This method involved PCR amplification systems and a hybridization platform.<BR><B>UNASSIGNED: </B>The technique can detect 13 hotspot mutations of 4 hearing loss-related genes. And a total of 213 blood samples were used to evaluate the availability of this method.<BR><B>UNASSIGNED: </B>Our reverse dot blot assay was a simple, rapid, accurate, and cost-effective method to identify hotspot mutations of 4 hearing loss-related genes in a Chinese population.