%0 Journal Article
%T Analysis of Protein Glycosylation in the ER.
%A Schoberer J
%A Shin YJ
%A Vavra U
%A Veit C
%A Strasser R
%J Methods Mol Biol
%V 2772
%N 0
%D 2024
%M 38411817
暂无%R 10.1007/978-1-0716-3710-4_16
%X Protein N-glycosylation is an essential posttranslational modification which is initiated in the endoplasmic reticulum (ER). In plants, the N-glycans play a pivotal role in protein folding and quality control. Through the interaction of glycan processing and binding reactions mediated by ER-resident glycosidases and specific carbohydrate-binding proteins, the N-glycans contribute to the adoption of a native protein conformation. Properly folded glycoproteins are released from these processes and allowed to continue their transit to the Golgi where further processing and maturation of N-glycans leads to the formation of more complex structures with different functions. Incompletely folded glycoproteins are removed from the ER by a highly conserved degradation process to prevent the accumulation or secretion of misfolded proteins and maintain ER homeostasis. Here, we describe methods to analyze the N-glycosylation status and the glycan-dependent ER-associated degradation process in plants.