%0 Journal Article
%T High-Throughput Screening for the Prevalence of Neutralizing Antibodies against Human Adenovirus Serotype 5.
%A Wettengel JM
%A Naka H
%A Dissen GA
%A Torgerson J
%A Pounder M
%A Mueller SF
%A Mueller E
%A Hagen P
%A Brandt M
%A Protzer U
%A Burwitz BJ
%J Vaccines (Basel)
%V 12
%N 2
%D 2024 Feb 1
%M 38400138
%F 4.961
%R 10.3390/vaccines12020155
%X Adenoviral vectors based on the human adenovirus species C serotype 5 (HAdV-C5) are commonly used for vector-based gene therapies and vaccines. In the preclinical stages of development, their safety and efficacy are often validated in suitable animal models. However, pre-existing neutralizing antibodies may severely influence study outcomes. Here, we generated a new HAdV-C5-based reporter vector and established a high-throughput screening assay for the multivalent detection of HAdV-C5-neutralizing antibodies in serum. We screened the sera of rhesus macaques at different primate centers, and of rabbits, horses, cats, and dogs, showing that HAdV-C5-neutralizing antibodies can be found in all species, albeit at different frequencies. Our results emphasize the need to prescreen model animals in HAdV-C5-based studies.