%0 Journal Article
%T Non-criteria antiphospholipid antibodies and calprotectin as potential biomarkers in pediatric antiphospholipid syndrome.
%A Sloan EE
%A Kmetova K
%A NaveenKumar SK
%A Kluge L
%A Chong E
%A Hoy CK
%A Yalavarthi S
%A Sarosh C
%A Baisch J
%A Walters L
%A Nassi L
%A Fuller J
%A Turnier JL
%A Pascual V
%A Wright TB
%A Madison JA
%A Knight JS
%A Zia A
%A Zuo Y
%J Clin Immunol
%V 261
%N 0
%D 2024 04 13
%M 38355030
%F 10.19
%R 10.1016/j.clim.2024.109926
%X Our study aimed to evaluate the presence, clinical associations, and potential mechanistic roles of non-criteria antiphospholipid antibodies (aPL) and circulating calprotectin, a highly stable marker of neutrophil extracellular trap release (NETosis), in pediatric APS patients. We found that 79% of pediatric APS patients had at least one non-criteria aPL at moderate-to-high titer. Univariate logistic regression demonstrated that positive anti-beta-2 glycoprotein I domain 1 (anti-D1) IgG (p = 0.008), anti-phosphatidylserine/prothrombin (aPS/PT) IgG (p < 0.001), and aPS/PT IgM (p < 0.001) were significantly associated with venous thrombosis. Positive anti-D1 IgG (p < 0.001), aPS/PT IgG (p < 0.001), and aPS/PT IgM (p = 0.001) were also associated with non-thrombotic manifestations of APS, such as thrombocytopenia. Increased levels of calprotectin were detected in children with APS. Calprotectin correlated positively with absolute neutrophil count (r = 0.63, p = 0.008) and negatively with platelet count (r = -0.59, p = 0.015). Mechanistically, plasma from pediatric APS patients with high calprotectin levels impaired platelet viability in a dose-dependent manner.