%0 Journal Article
%T RNA-binding protein IGF2BP2 suppresses metastasis of clear cell renal cell carcinoma by enhancing CKB mRNA stability and expression.
%A Ren J
%A Huang B
%A Li W
%A Wang Y
%A Pan X
%A Ma Q
%A Liu Y
%A Wang X
%A Liang C
%A Zhang Y
%A Wang S
%A Yang F
%A Li H
%A Ning H
%A Jiang Y
%A Qin C
%A Ran A
%A Xiao B
%J Transl Oncol
%V 42
%N 0
%D 2024 Apr 10
%M 38341962
%F 4.803
%R 10.1016/j.tranon.2024.101904
%X Clear cell renal cell carcinoma (ccRCC) is the most prevalent kidney cancer, with a highly aggressive phenotype and poor prognosis. RNA binding proteins (RBPs) play crucial roles in post-transcriptional gene regulation and have been implicated in tumorigenesis. RBPs have the potential to become a new therapeutic target for ccRCC. In this study, we screened and validated that insulin-like growth factor 2 mRNA binding protein 2 (IGF2BP2) as an RBP, was down-regulated in ccRCC tissues and cell lines. Functionally, we verified that IGF2BP2 significantly suppressed the migration and invasion ability of ccRCC in vitro and in vivo. Mechanistically, RIP-seq and actinomycin D experiments results showed that IGF2BP2 enhanced the expression of Creatine Kinase B (CKB) by binding to CKB mRNA and enhancing its mRNA stability. Thus, IGF2BP2 inhibited ccRCC metastasis through enhancing the expression of CKB. Taken together, these finding suggests that IGF2BP2 is a novel metastasis suppressor of ccRCC and may serve as a potential therapeutic target.