%0 Journal Article
%T Plasma ddPCR for the detection of MET amplification in advanced NSCLC patients: a comparative real-world study.
%A Su JW
%A Weng CD
%A Lin XC
%A Fang MM
%A Xiao X
%A Zhang YC
%A Zhang XC
%A Su J
%A Xu CR
%A Yan HH
%A Chen HJ
%A Wu YL
%A Yang JJ
%J Ther Adv Med Oncol
%V 16
%N 0
%D 2024
%M 38333112
%F 5.485
%R 10.1177/17588359241229435
%X <B>UNASSIGNED: </B>Mesenchymal-epithelial transition (MET) amplification is a crucial oncogenic driver and a resistance mechanism to epidermal growth factor receptor tyrosine kinase inhibitors (TKIs) of non-small-cell lung cancer (NSCLC). Fluorescence in situ hybridization (FISH) is the gold standard for MET amplification detection. However, it is inapplicable when tissue samples are unavailable.<BR><B>UNASSIGNED: </B>This study assessed the performance of plasma droplet digital polymerase chain reaction (ddPCR) in MET amplification detection in NSCLC patients.<BR><B>UNASSIGNED: </B>A total of 87 NSCLC patients were enrolled, and 94 paired tissue and plasma samples were analyzed for the concordance between FISH and plasma ddPCR/tissue next-generation sequencing (NGS) in detecting MET amplification. In addition, the efficacy of patients with MET amplification using different detection methods who were treated with MET-TKIs was evaluated.<BR><B>UNASSIGNED: </B>Plasma ddPCR showed substantial concordance with FISH (74.1% sensitivity, 92.5% specificity, and 87.2% accuracy with a kappa value of 0.68) and outperformed tissue NGS (kappa value of 0.64) in MET amplification detection. Combined plasma ddPCR and tissue NGS showed substantial concordance with FISH (92.3% sensitivity, 89.2% specificity, and an accuracy of 90.1% with a kappa value of 0.77). The efficacy is comparable in these NSCLC patients with MET amplification detected by FISH and plasma ddPCR who were treated with MET-TKIs.<BR><B>UNASSIGNED: </B>Plasma ddPCR is a potentially reliable method for detecting MET amplification in advanced NSCLC patients. Combined plasma ddPCR and tissue NGS might be an alternative or complementary method to MET amplification detection.