%0 Journal Article
%T Assessing polyglutamine tract aggregation in the nematode Caenorhabditis elegans.
%A Sotiriou A
%A Ploumi C
%A Charmpilas N
%A Tavernarakis N
%J Methods Cell Biol
%V 181
%N 0
%D 2024
%M 38302233
%F 1.829
%R 10.1016/bs.mcb.2022.09.003
%X Proteome integrity is a prerequisite for cellular functionality and organismal viability. Its compromise is considered an inherent part of the aging process and has been associated with the onset of age-related, neurodegenerative pathologies. Although the molecular underpinnings of protein homeostasis (proteostasis) have been extensively studied, several aspects of its regulation remain elusive. The nematode Caenorhabditis elegans has emerged as a versatile, heterologous model organism to study the dynamics of aggregation-prone human proteins in vivo. Here, we describe an experimental pipeline for the analysis of polyglutamine (polyQ) tract aggregation, as a measure of the state of proteostasis, during aging.