%0 Journal Article
%T Effect and mechanism of recombinant human fibroblast growth factor 18 on osteoporosis in OVX mice.
%A Lu PY
%A Huang M
%A Shao MH
%A Hu JX
%A Ding CY
%A Feng YJ
%A Zhang M
%A Lin HP
%A Tian HS
%J Climacteric
%V 27
%N 3
%D 2024 Jun 26
%M 38275172
%F 3.024
%R 10.1080/13697137.2024.2302967
%X <B>UNASSIGNED: </B>This study aimed to investigate the effect and the mechanism of recombinant human fibroblast growth factor 18 (rhFGF18) on postmenopausal osteoporosis.<BR><B>UNASSIGNED: </B>The effect of rhFGF18 on the proliferation and apoptosis of osteoblasts and the mechanism underlying such an effect was evaluated using an oxidative stress model of the MC3T3-E1 cell line. Furthermore, ovariectomy was performed on ICR mice to imitate estrogen-deficiency postmenopausal osteoporosis. Bone metabolism and bone morphological parameters in the ovariectomized (OVX) mice were evaluated.<BR><B>UNASSIGNED: </B>The results obtained from the cell model showed that FGF18 promoted MC3T3-E1 cell proliferation by activating the extracellular signal-regulated kinase (ERK) and p38 instead of c-Jun N-terminal kinase (JNK). FGF18 also prevented cells from damage inflicted by oxidative stress via inhibition of apoptosis. After FGF18 administration, the expression level of anti-apoptotic protein Bcl-2 in the mice was upregulated, whereas those of the pro-apoptotic proteins Bax and caspase-3 were downregulated. Administering FGF18 also improved bone metabolism and bone morphological parameters in OVX mice.<BR><B>UNASSIGNED: </B>FGF18 could effectively prevent bone loss in OVX mice by enhancing osteoblastogenesis and protecting osteoblasts from oxidative stress-induced apoptosis.