%0 Journal Article
%T High-throughput CRISPR technology: a novel horizon for solid organ transplantation.
%A Li X
%A Chen Z
%A Ye W
%A Yu J
%A Zhang X
%A Li Y
%A Niu Y
%A Ran S
%A Wang S
%A Luo Z
%A Zhao J
%A Hao Y
%A Zong J
%A Xia C
%A Xia J
%A Wu J
%J Front Immunol
%V 14
%N 0
%D 2023
%M 38239344
%F 8.786
%R 10.3389/fimmu.2023.1295523
%X Organ transplantation is the gold standard therapy for end-stage organ failure. However, the shortage of available grafts and long-term graft dysfunction remain the primary barriers to organ transplantation. Exploring approaches to solve these issues is urgent, and CRISPR/Cas9-based transcriptome editing provides one potential solution. Furthermore, combining CRISPR/Cas9-based gene editing with an ex vivo organ perfusion system would enable pre-implantation transcriptome editing of grafts. How to determine effective intervention targets becomes a new problem. Fortunately, the advent of high-throughput CRISPR screening has dramatically accelerated the effective targets. This review summarizes the current advancements, utilization, and workflow of CRISPR screening in various immune and non-immune cells. It also discusses the ongoing applications of CRISPR/Cas-based gene editing in transplantation and the prospective applications of CRISPR screening in solid organ transplantation.