%0 Journal Article
%T The Current Situation and Development Prospect of Whole-Genome Screening.
%A Yang C
%A Lei Y
%A Ren T
%A Yao M
%J Int J Mol Sci
%V 25
%N 1
%D 2024 Jan 4
%M 38203828
%F 6.208
%R 10.3390/ijms25010658
%X High-throughput genetic screening is useful for discovering critical genes or gene sequences that trigger specific cell functions and/or phenotypes. Loss-of-function genetic screening is mainly achieved through RNA interference (RNAi), CRISPR knock-out (CRISPRko), and CRISPR interference (CRISPRi) technologies. Gain-of-function genetic screening mainly depends on the overexpression of a cDNA library and CRISPR activation (CRISPRa). Base editing can perform both gain- and loss-of-function genetic screening. This review discusses genetic screening techniques based on Cas9 nuclease, including Cas9-mediated genome knock-out and dCas9-based gene activation and interference. We compare these methods with previous genetic screening techniques based on RNAi and cDNA library overexpression and propose future prospects and applications for CRISPR screening.