%0 Journal Article
%T HIC1 interacts with FOXP3 multi protein complex: Novel pleiotropic mechanisms to regulate human regulatory T cell differentiation and function.
%A Andrabi SBA
%A Batkulwar K
%A Bhosale SD
%A Moulder R
%A Khan MH
%A Buchacher T
%A Khan MM
%A Arnkil I
%A Rasool O
%A Marson A
%A Kalim UU
%A Lahesmaa R
%J Immunol Lett
%V 263
%N 0
%D 2023 11 12
%M 37838026
%F 4.23
%R 10.1016/j.imlet.2023.09.001
%X Transcriptional repressor, hypermethylated in cancer 1 (HIC1) participates in a range of important biological processes, such as tumor repression, immune suppression, embryonic development and epigenetic gene regulation. Further to these, we previously demonstrated that HIC1 provides a significant contribution to the function and development of regulatory T (Treg) cells. However, the mechanism by which it regulates these processes was not apparent. To address this question, we used affinity-purification mass spectrometry to characterize the HIC1 interactome in human Treg cells. Altogether 61 high-confidence interactors were identified, including IKZF3, which is a key transcription factor in the development of Treg cells. The biological processes associated with these interacting proteins include protein transport, mRNA processing, non-coding (ncRNA) transcription and RNA metabolism. The results revealed that HIC1 is part of a FOXP3-RUNX1-CBFB protein complex that regulates Treg signature genes thus improving our understanding of HIC1 function during early Treg cell differentiation.