%0 Journal Article
%T Ultra-Fast Single-Nucleotide-Variation Detection Enabled by Argonaute-Mediated Transistor Platform.
%A Kong D
%A Zhang S
%A Guo M
%A Li S
%A Wang Q
%A Gou J
%A Wu Y
%A Chen Y
%A Yang Y
%A Dai C
%A Tian Z
%A Wee ATS
%A Liu Y
%A Wei D
%J Adv Mater
%V 36
%N 5
%D 2024 Feb 8
%M 37805919
%F 32.086
%R 10.1002/adma.202307366
%X "Test-and-go" single-nucleotide variation (SNV) detection within several minutes remains challenging, especially in low-abundance samples, since existing methods face a trade-off between sensitivity and testing speed. Sensitive detection usually relies on complex and time-consuming nucleic acid amplification or sequencing. Here, a graphene field-effect transistor (GFET) platform mediated by Argonaute protein that enables rapid, sensitive, and specific SNV detection is developed. The Argonaute protein provides a nanoscale binding channel to preorganize the DNA probe, accelerating target binding and rapidly recognizing SNVs with single-nucleotide resolution in unamplified tumor-associated microRNA, circulating tumor DNA, virus RNA, and reverse transcribed cDNA when a mismatch occurs in the seed region. An integrated microchip simultaneously detects multiple SNVs in agreement with sequencing results within 5 min, achieving the fastest SNV detection in a "test-and-go" manner without the requirement of nucleic acid extraction, reverse transcription, and amplification.