%0 Journal Article
%T Bradykinin deficiency causes high blood pressure in mice.
%A Ohnaka Y
%A Tsukamoto S
%A Iwai Y
%A Hamada-Kanazawa M
%A Kariya R
%A Takano M
%J Biochem Biophys Res Commun
%V 681
%N 0
%D 2023 11 12
%M 37757669
%F 3.322
%R 10.1016/j.bbrc.2023.09.059
%X Bradykinin has a wide variety of physiological functions, including vasodilation and blood pressure reduction. However, the physiological roles of bradykinin are not fully understood. We used the CRISPR/Cas9 method to generate BKdelK1 and BKdelK2 mutant mice, targeting the BK portion of mouse kininogen1 and kininogen2 genes, respectively. The BKdelK1 and BKdelK2 mutant mice had about 50% reductions in plasma low molecular weight kininogen and trypsin-released BK, compared to wild mice. Both BKdelK1 and BKdelK2 mice had significantly elevated systolic blood pressure compared to WT mice. These results suggest that plasma LKNG is a source of KNG in the vascular kallikrein-kinin system and contributes to maintaining lower systolic blood pressure.