%0 Journal Article
%T Antibiotic that inhibits trans-translation blocks binding of EF-Tu to tmRNA but not to tRNA.
%A Marathe N
%A Nguyen HA
%A Alumasa JN
%A Kuzmishin Nagy AB
%A Vazquez M
%A Dunham CM
%A Keiler KC
%J mBio
%V 14
%N 5
%D 2023 Oct 31
%M 37681945
暂无%R 10.1128/mbio.01461-23
%X <B>OBJECTIVE: </B>Elongation factor thermo-unstable (EF-Tu) is a universally conserved translation factor that mediates productive interactions between tRNAs and the ribosome. In bacteria, EF-Tu also delivers transfer-messenger RNA (tmRNA)-SmpB to the ribosome during trans-translation. We report the first small molecule, KKL-55, that specifically inhibits EF-Tu activity in trans-translation without affecting its activity in normal translation. KKL-55 has broad-spectrum antibiotic activity, suggesting that compounds targeted to the tmRNA-binding interface of EF-Tu could be developed into new antibiotics to treat drug-resistant infections.