%0 Video-Audio Media
%T Genetic reporter for live tracing fluid flow forces during cell fate segregation in mouse blastocyst development.
%A Zhang Y
%A Li X
%A Gao S
%A Liao Y
%A Luo Y
%A Liu M
%A Bian Y
%A Xiong H
%A Yue Y
%A He A
%J Cell Stem Cell
%V 30
%N 8
%D 2023 08 3
%M 37541214
%F 25.269
%R 10.1016/j.stem.2023.07.003
%X Mechanical forces are known to be important in mammalian blastocyst formation; however, due to limited tools, specific force inputs and how they relay to first cell fate control of inner cell mass (ICM) and/or trophectoderm (TE) remain elusive. Combining in toto live imaging and various perturbation experiments, we demonstrate and measure fluid flow forces existing in the mouse blastocyst cavity and identify Klf2(Krüppel-like factor 2) as a fluid force reporter with force-responsive enhancers. Long-term live imaging and lineage reconstructions reveal that blastomeres subject to higher fluid flow forces adopt ICM cell fates. These are reinforced by internal ferrofluid-induced flow force assays. We also utilize ex vivo fluid flow force mimicking and pharmacological perturbations to confirm mechanosensing specificity. Together, we report a genetically encoded reporter for continuously monitoring fluid flow forces and cell fate decisions and provide a live imaging framework to infer force information enriched lineage landscape during development. VIDEO ABSTRACT.