%0 Journal Article
%T Identification of Chimeric NTRK3 Genes in Papillary Thyroid Cancer Cells by Analyzing the Imbalance of the Expression of 5' and 3' mRNA Fragments.
%A Oskorbin IP
%A Ivanov AA
%A Smertina MA
%A Demidova IA
%A Boyarskikh UA
%A Kechin AA
%A Bakharev SY
%A Samuilenkova OV
%A Vihlyanov IV
%A Kushlinskii NE
%A Filipenko ML
%J Bull Exp Biol Med
%V 175
%N 2
%D 2023 Jun 21
%M 37477743
%F 0.737
%R 10.1007/s10517-023-05842-x
%X The standard for detecting chimeric genes of neurotrophic receptor tyrosine kinases (NTRK) is next generation sequencing (NGS). However, this analysis is expensive and takes several days. As a rapid screening method for the detection of NTRK3-dependent papillary thyroid cancer, an analysis of the expression imbalance between 5' and 3' NTRK3 mRNA fragments was used (5'/3' RT-PCR). The reference method for detection of NTRK3 rearrangements was fluorescent in situ hybridization (FISH), and the most frequent rearrangements in papillary thyroid cancer were tested using reverse transcription PCR (RT-PCR). Using 5'/3' RT-PCR, 18 samples of papillary thyroid cancer carrying chimeric transcripts of NTRK3 mRNA were detected. The sensitivity of the developed technique was 88.9% and specificity was 99.3%. Thus, a fast and cost-effective method of screening samples of papillary thyroid cancer in paraffin blocks is proposed with acceptable sensitivity and specificity.