%0 Journal Article
%T In vitro study and pharmacokinetic evaluation of sitagliptin phosphate enantiomers in rat plasma.
%A Spandana T
%A Goli VV
%A Gurupadayya B
%J Bioanalysis
%V 15
%N 16
%D 2023 Aug
%M 37431826
%F 2.695
%R 10.4155/bio-2023-0084
%X Background: A chiral HPLC technique was developed to determine sitagliptin phosphate enantiomers in rat plasma in compliance with US FDA regulations. Methods & results: The technique used a Phenomenex column with a mobile phase consisting of a 60:35:5 (v/v/v) blend of pH4, 10-mM ammonium acetate buffer, methanol and 0.1% formic acid in Millipore water. The precision for both (R) and (S) sitagliptin phosphate varied between 0.246 and 1.246%, while the accuracy was 99.6-100.1%. A glucose uptake assay was used to assess enantiomers in 3T3-L1 cell lines through flow cytometry. Conclusion: Investigation of the pharmacokinetic impacts of sitagliptin phosphate racemic enantiomers in rat plasma revealed notable contrasts in R and S enantiomers in female albino Wistar rats, suggesting enantioselectivity for sitagliptin phosphate.