%0 Journal Article
%T Whole-Mount In Situ Hybridization in Large Sexual Schmidtea mediterranea.
%A Vila-Farré M
%A Vu HT
%A Rink JC
%J Methods Mol Biol
%V 2680
%N 0
%D 2023
%M 37428374
暂无%R 10.1007/978-1-0716-3275-8_7
%X Whole-mount in situ hybridization (WISH), colorimetric or fluorescent (FISH), allows for the visualization of endogenous RNA. For planarians, robust WISH protocols exist for small-sized animals (>5 mm) of the model species Schmidtea mediterranea and Dugesia japonica. However, the sexual strain of Schmidtea mediterranea studied for germline development and function reaches much larger body sizes in excess of 2 cm. The existing whole-mount WISH protocols are not optimal for such large specimens, owing to insufficient tissue permeabilization. Here, we describe a robust WISH protocol for 12-16 mm long sexually mature Schmidtea mediterranea individuals that could serve as a starting point for adapting WISH to other large planarian species.