%0 Journal Article
%T A differentiation protocol for the generation of pancreatic beta-like cells from human embryonic stem cells.
%A Li X
%A Ma Z
%A Lui KO
%J STAR Protoc
%V 4
%N 3
%D 2023 Sep 15
%M 37392391
暂无%R 10.1016/j.xpro.2023.102407
%X By virtue of their capability to replicate and regenerate, human stem-cell-derived beta-like cells could be a valuable resource for cellular therapy targeting insulin-dependent diabetes. Here, we present a protocol to generate beta-like cells from human embryonic stem cells (hESCs). We first describe steps for differentiation of beta-like cells from hESCs and CD9-negative beta-like cell enrichment through fluorescence-activated cell sorting. We then detail immunofluorescence, flow cytometry, and glucose-stimulated insulin secretion assay for characterization of human beta-like cells. For complete details on the use and execution of this protocol, please refer to Li et al. (2020).1.