%0 Journal Article
%T Recruitment of transcription factor ETS1 to activated accessible regions promotes the transcriptional program of cilia genes.
%A Zhang D
%A Zhang C
%A Zhu Y
%A Xie H
%A Yue C
%A Li M
%A Wei W
%A Peng Y
%A Yin G
%A Guo Y
%A Guan Y
%J Nucleic Acids Res
%V 51
%N 13
%D 2023 07 21
%M 37326025
%F 19.16
%R 10.1093/nar/gkad506
%X Defects in cilia genes, which are critical for cilia formation and function, can cause complicated ciliopathy syndromes involving multiple organs and tissues; however, the underlying regulatory mechanisms of the networks of cilia genes in ciliopathies remain enigmatic. Herein, we have uncovered the genome-wide redistribution of accessible chromatin regions and extensive alterations of expression of cilia genes during Ellis-van Creveld syndrome (EVC) ciliopathy pathogenesis. Mechanistically, the distinct EVC ciliopathy-activated accessible regions (CAAs) are shown to positively regulate robust changes in flanking cilia genes, which are a key requirement for cilia transcription in response to developmental signals. Moreover, a single transcription factor, ETS1, can be recruited to CAAs, leading to prominent chromatin accessibility reconstruction in EVC ciliopathy patients. In zebrafish, the collapse of CAAs driven by ets1 suppression subsequently causes defective cilia proteins, resulting in body curvature and pericardial oedema. Our results depict a dynamic landscape of chromatin accessibility in EVC ciliopathy patients, and uncover an insightful role for ETS1 in controlling the global transcriptional program of cilia genes by reprogramming the widespread chromatin state.