%0 Journal Article
%T STAT3 signaling in B cells controls germinal center zone organization and recycling.
%A Fike AJ
%A Chodisetti SB
%A Wright NE
%A Bricker KN
%A Domeier PP
%A Maienschein-Cline M
%A Rosenfeld AM
%A Luckenbill SA
%A Weber JL
%A Choi NM
%A Luning Prak ET
%A Mandal M
%A Clark MR
%A Rahman ZSM
%J Cell Rep
%V 42
%N 5
%D 2023 05 30
%M 37200190
暂无%R 10.1016/j.celrep.2023.112512
%X Germinal centers (GCs), sites of antibody affinity maturation, are organized into dark (DZ) and light (LZ) zones. Here, we show a B cell-intrinsic role for signal transducer and activator of transcription 3 (STAT3) in GC DZ and LZ organization. Altered zonal organization of STAT3-deficient GCs dampens development of long-lived plasma cells (LL-PCs) but increases memory B cells (MBCs). In an abundant antigenic environment, achieved here by prime-boost immunization, STAT3 is not required for GC initiation, maintenance, or proliferation but is important for sustaining GC zonal organization by regulating GC B cell recycling. Th cell-derived signals drive STAT3 tyrosine 705 and serine 727 phosphorylation in LZ B cells, regulating their recycling into the DZ. RNA sequencing (RNA-seq) and chromatin immunoprecipitation sequencing (ChIP-seq) analyses identified STAT3 regulated genes that are critical for LZ cell recycling and transiting through DZ proliferation and differentiation phases. Thus, STAT3 signaling in B cells controls GC zone organization and recycling, and GC egress of PCs, but negatively regulates MBC output.