%0 Randomized Controlled Trial
%T A randomized double-blind controlled proof-of-concept study of alanyl-glutamine for reduction of post-myomectomy adhesions.
%A Chizen DR
%A Rislund DC
%A Robertson LM
%A Lim HJ
%A Tulandi T
%A Gargiulo AR
%A De Wilde RL
%A Velygodskiy A
%A Pierson RA
%J Eur J Obstet Gynecol Reprod Biol
%V 284
%N 0
%D May 2023
%M 37023559
%F 2.831
%R 10.1016/j.ejogrb.2023.03.032
%X <B>OBJECTIVE: </B>To test the hypothesis that intraperitoneal instillation of a single bolus dose of l-alanyl-l-glutamine (AG) will reduce the incidence, extent and/or severity of adhesions following myomectomy and establish preliminary safety and tolerability of AG in humans.<BR><B>METHODS: </B>Phase 1,2 Randomized, double-blind, placebo-controlled study (DBRCT).<BR><B>METHODS: </B>Tertiary care gynecology surgical centre.<BR><B>METHODS: </B>Thirty-eight women who underwent myomectomies by laparoscopy (N = 38; AG-19 vs Placebo-19) or laparotomy (N = 10; AG-5 vs Placebo-5) with a scheduled second-look laparoscopy (SLL) 6-8 weeks later. Thirty-two patients in the laparoscopy arm completed SLL.<BR><B>METHODS: </B>Bolus dose of AG or normal saline solution control (0.9% NaCl) administered intraperitoneally immediately prior to suture closure of the laparoscopic ports. The average dose was 170 mL of AG or control based on a dosing scheme of 1 g/kg bodyweight.<BR><B>METHODS: </B>Digital recordings obtained for all procedures. The primary endpoint was reduction in the incidence, severity and extent of post-operative adhesions analyzed by intention-to-treat (ITT) approach. Three independent, blinded reviewers evaluated all operative video recordings to assess presence of adhesions. Post-hoc analysis assessed presence or absence of adhesions in the peritoneal cavity. Secondary endpoints assessed safety and tolerability of AG.<BR><B>RESULTS: </B>Administration of AG reduced the incidence, severity and/or extent of post-operative adhesions (p = 0.046). The presence of adhesions in the AG group was lower than in the Control group (p = 0.041). Adhesion improvement was achieved in 15 of 15 (100%) in the AG group versus 5 of 17 (29.6%) in the placebo group. No serious adverse events were reported. No differences in safety parameters were observed.<BR><B>CONCLUSIONS: </B>Intraperitoneal l-alanyl-l-glutamine reduced adhesion formation in all patients following laparoscopic myomectomy. Complete absence of adhesions was achieved at all abdominal sites in 93% of patients. Results confirm AG's known effects on cellular mechanisms of adhesiogenesis and lay the foundation for new adhesion prophylaxis research and treatment.