%0 Journal Article
%T Monitoring the Release of Methylglyoxal (MGO) from Honey and Honey-Based Formulations.
%A Hossain ML
%A Lim LY
%A Hammer K
%A Hettiarachchi D
%A Locher C
%J Molecules
%V 28
%N 6
%D Mar 2023 22
%M 36985830
%F 4.927
%R 10.3390/molecules28062858
%X Methylglyoxal (MGO) is considered to be one of the vital components responsible for the anti-bacterial activity of Leptospermum spp. (Manuka) honey. While many studies have demonstrated a dose-dependent antibacterial activity for MGO in vitro, from a therapeutic viewpoint, it is also important to confirm its release from Manuka honey and also from Manuka honey-based formulations. This study is the first to report on the release profile of MGO from five commercial products containing Manuka honey using a Franz diffusion cell and High-Performance Liquid Chromatography (HPLC) analysis. The release of MGO expressed as percentage release of MGO content at baseline was monitored over a 12 h period and found to be 99.49 and 98.05% from an artificial honey matrix and NZ Manuka honey, respectively. For the investigated formulations, a time-dependent % MGO release between 85% and 97.18% was noted over the 12 h study period.