%0 Journal Article
%T Identification of the viral and cellular microRNA interactomes during SARS-CoV-2 infection.
%A Fossat N
%A Lundsgaard EA
%A Costa R
%A Rivera-Rangel LR
%A Nielsen L
%A Mikkelsen LS
%A Ramirez S
%A Bukh J
%A Scheel TKH
%J Cell Rep
%V 42
%N 4
%D 04 2023 25
%M 36961814
暂无%R 10.1016/j.celrep.2023.112282
%X The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic has had a tremendous impact worldwide. Mapping virus-host interactions is critical to understand disease progression. MicroRNAs (miRNAs) are important RNA regulators, but their interaction with SARS-CoV-2 RNA was not experimentally investigated. Here, using Argonaute (AGO) cross-linking immunoprecipitation combined with RNA proximity ligation (CLEAR-CLIP), we provide unbiased mapping of SARS-CoV-2/miRNA interactions. We identified six main regions on the viral RNA bound primarily by one specific miRNA. Targeted mutagenesis and AGO1-3 knockdown demonstrated that these interactions are not critical for virus production. Moreover, we identified perturbed regulation of cellular miRNA interactions during infection, including non-compensated viral sequestration of the miR-15 family. Transcriptome analysis further showed that mRNAs targeted by this miRNA family are derepressed. This work delineates the interphase between miRNA regulation and SARS-CoV-2 infection and further contributes to deciphering the full molecular interactome of this virus.