%0 Journal Article
%T Development of an efficient method for selection of stable cell pools for protein expression and surface display with Expi293F cells.
%A Chen C
%A Wang Z
%A Sun Z
%A Li W
%A Dimitrov DS
%J Cell Biochem Funct
%V 41
%N 3
%D Apr 2023
%M 36864545
%F 3.963
%R 10.1002/cbf.3787
%X Compare with transient expression, stable cell lines generally have higher productivity and better quality for protein expression. However, selection of stable cell line is time-consuming and laborious. Here we describe an optimized selection method to achieve high-efficient stable cell pools with Expi293F suspension cells. This method only takes 2-3 weeks to generate stable cell pools with 2- to 10-fold higher productivity than transient gene expression (TGE). In fed-batch culture with Yeastolate, >1 g/L yield was achieved with our KTN0239-IgG stable cell pool in shaker flasks. This method can be also applied to efficiently display proteins on the cell surface.